By applying your Ph. D. program, I am seeking to fulfill a dream in my childhood, to be a great scientist. This dream encouraged me to read a lot of books on many disciplines, since my primary school time. Although my parents had to work abroad much of the time from then on and had no time to look into my study, I had never been slack, for the dream gave me a pressure to do my best in study.
通過申請貴校的博士生，我將要實現(xiàn)我童年時的夢想-成為一名偉大的科學家。自小學以來，這個夢想就鼓勵著我去閱讀許多學科專業(yè)的書籍。盡管在那以后，我的父母不得不大部分時間在國外工作而且沒有時間關心我的學習，但是我從沒有松懈過，因為夢想給我壓力，讓我在學業(yè)上奮力拼搏。
In the meanwhile, my talent in mathematics helped me excel my classmates and gain outstanding grades. Besides my excellent grades in school, I won remarkable scores in various contests in Beijing and a few national contests on mathematics, physics, chemistry, etc. Moreover, from 1993 to 1998, I won the Shupin Scholarship several times based on my excellent score.
 
When I was in high school, I came to realize the great potential of life sciences. As we all know, mankind can go up to outer space or down to the bottom of the ocean, and predict the movement or state of a huge planet or an infinitesimal particle. Yet we can’t explain a lot of phenomena about ourselves or various lives around us, because lives are the most complicated systems in the world. I am sure that to explain these phenomena is what a great scientist should do, and just what I wish to do.
 
Therefore, when I was about to take the National College Entrance Examination, I chose the college of Life Sciences in Peking University, the most famous university in China, which is dubbed as China's Harvard by President Clinton on his visit to my country. In the university, I studied at the Department of Biochemistry & Molecular Biology, which required its students to have a great ability to learn and research. With the study of a series of courses, I obtained a solid academic background in chemistry and basic biology. At the same time, I learned many skills of both general chemistry experiments and specialized biology research. From lab courses, I experienced all kinds of experiments for DNA, protein and microbe’s research and analysis.
 
However, I realized that I need more practical experience before I step on the way to be a scientist. To enhance my research ability, I have been working as a research assistant since July 2000 in the Laboratory of Molecular Virology & Bioengineering at the Institute of Microbiology, Chinese Academy of Sciences (CAS) for a project on the regulation of cotton fertility in plant heterosis, which is part of the National High Technology Research & Development Program (the “863 Program”), and have got great progress in the work of clone, absence and analysis of promoter’s function on the specific expressed gene in cotton pollen. We constructed plasmids that have pollen specific promoters and poisonous protein genes, and then transformed the recombinant genes into tobacco by agrobacterium or particle bombardment to study the long time and short time characteristics of them and their mutants under different conditions.
 
At the same time, I expressed a nuclease inhibitor gene, and purified the protein to prepare its antibody. With the antibody, I succeed in screening transformed cottons by immunoassays. This method can greatly improve the application of cotton’s heterosis, which has potential significance in our country’s cotton production. With some of the work above, I completed my undergraduate thesis, “High level expression of a nuclease inhibitor, Bastar in E.Coli and its application in screening transformed cotton”, and got a good score.
 
In addition, my skills gave me chances to be appointed to participate in the jobs of other projects, such as the study on the promoter and its function of specific expressed Annexin in strawberry fruit, and the translation of a book on Prion Biology and Disease. These jobs, which are in progress, gave me more exercises in various areas.
 
These studies and research experience provided me the first step to the way of being a biology scientist. Because they not only furthered my learning and experimental skills, but also greatly increased my interest in the field of Regulation of Gene Expression and its application in Protein's Structure and Function. I would like to continue my study in molecular biology and biochemistry, especially in the area of gene regulation, study on the character of DNA and protein, as well as DNA-protein interaction. Because I think they are the foundations of life sciences and involved in nearly every biological process. Both DNA and protein are the most important part and the most mysterious part of life. Many problems about them remained to be explained. This provided opportunity for me to do research for the progress of life sciences. Certainly, the application in Public Health is also what I concern and I’ve read many works on it during my research work.
 
Besides academic study and experimental training, I also benefited a lot from the social work I have done during my college life. I led a group of volunteers to provide service to the guests on the occasion of the 100th Anniversary of Peking University. We did a good job and thus were praised by the supervisor. With this experience, my team spirit and leadership, which may contribute a lot to my future research, have been greatly improved.
 
This summer, I’ll graduate from Peking University and I’d like to further my study in a college with better research doctrine and advanced experimental facilities, which your university can provide me. Moreover, you have the conditions to contact with most advanced progress of biology in the world. I have the confidence that, with my assiduity and the favorable conditions provided by your university, I can make full use of my talent and become a great scientist in life sciences. So, why don’t you choose me and give me a chance to fulfill my dream?
 
Sincerely yours,
 
xxxx
 
Department of Biochemistry & Molecular Biology
College of Life Sciences
****University
P. R. China